Application of VIGS system to explore the function of common wheat TaADF7

As an allohexaploid plant, common wheat has a complex gene structure, making it difficult to study its function. Virus-induced silencing (VIGS) is important tool for the rapid analysis of plant In this study, system, namely, barley stripe mosaic virus (BSMV)-VIGS induced by BSMV was used silence phytoene desaturase (TaPDS) and actin depolymerization factor (TaADF7) genes determine effect on wheat. TaPDS as indicator feasibility VIGS while TaADF7 test growth. Results showed that leaves tobacco were bleached mixture pCaBS-α, pCaBS-β, pCaBS-γ::TaPDS, indicating silenced, had physiological activity determined trypan blue staining. Therefore, system efficient available. After treated with pCaBS-γ::TaADF7, viral suspension obtained. The expression downregulated after infected friction, silenced. Laser confocal scanning microscopy enhanced fluorescence microfilament skeleton in mesophyll protoplasts significantly reduced height. affected cell division growth inhibiting depolymerization. conclusion, BSMV-VIGS genes. Bleaching phenomenon observed silencing. silencing, microfilaments cells gathered into coarse bundles, which dynamics inhibited